YFP (mVenus) Lentivirus

YFP (mVenus) Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain a YFP (yellow fluorescent protein) under the control of an EF1a promoter. The lentiviruses also transduce a puromycin selection marker (Figure 1). YFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry. YFP (mVenus) has an excitation wavelength of 515 nm, an emission wavelength of 528 nm, with an extinction coefficient of 104,000 M-1cm-1. Fluorescent proteins have been extensively used in cell biology studies. The most commonly used fluorescent proteins are derived from the Aequorea Victoria GFP (green fluorescent protein). Engineering of the chromophore-forming amino acids can result in different absorption and emission characteristics. YFPs (yellow fluorescent proteins) have a shift towards yellow, giving scientists the flexibility to use multiple colors.