STAT4 Luciferase Reporter Lentivirus (GAS-IRF-1)

STAT4 Luciferase Reporter Lentivirus (GAS-IRF-1) replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce most mammalian cells, including primary and non-dividing cells. These viruses contain a firefly luciferase reporter driven by multiple copies of interferon-gamma-activated sequence of the interferon regulatory factor 1 promoter (GAS of IRF1) located upstream of the minimal TATA promoter. The lentiviruses also contain a puromycin selection marker (Figure 1). STAT4 was found to bind to this GAS element of IRF-1 following stimulation of cells with IL-12 (interleukin 12). The lentiviruses also contain a puromycin selection marker (Figure 1). Note: To study the IL-12 induced STAT4 (Signal transducer and activator of transcription 4) signaling pathway, STAT4 and IL-12 receptor may also need to be introduced, depending on the target cells. Figure 1. Schematic of the lenti-vector used to generate the STAT4 Luciferase Reporter Lentivirus (GAS-IRF-1).