Nuclear eGFP Lentivirus (Puromycin)

Nuclear eGFP Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain a nuclear eGFP (enhanced green fluorescent protein), with nuclear localization sequences (NLS) at both N and C-terminus of eGFP), under the control of an EF1a promoter. The lentiviruses also transduce a puromycin selection marker (Figure 1). eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry. eGFP has an excitation wavelength of 488 nm, an emission wavelength of 509 nm, with an extinction coefficient of 55,000 M-1cm-1.