CD8a/CD8b Lentivirus

CD8 (cluster of differentiation 8) is a cell surface glycoprotein found on most cytotoxic T lymphocytes that functions within the immune system to mediate cell-cell interactions. The functional forms of CD8 consist of either a heterodimer of two isoforms, CD8alpha and CD8beta, or a homodimer of two CD8alpha molecules. CD8 acts as a coreceptor to facilitate binding between the T-cell Receptor (TCR) and the class I Major Histocompatibility Complex (MHC). Studying CD8 and its importance in isoimmunity can further our understanding of post-transplant recognition. The CD8a/CD8b (CD8alpha/CD8beta) Lentiviruses are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. The particles contain P2A-linked CD8a (NM_001768.6) and CD8b (NM_004931.5) driven by an EF1a promoter and a puromycin selection marker).