B2M HLA-B*40:01 Lentivirus

B2M HLA-B*40:01 Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce most mammalian cells, including primary and non-dividing cells. These viruses result in the expression of B2M (beta-2 microglobulin) HLA (human leukocyte antigen)-B*40:01 driven by an EF1a promoter. The lentiviruses also contain a puromycin selection marker (Figure 1). Figure 1. Schematic of the lenti-vector used to generate B2M HLA-B*40:01 Lentivirus. Human Leukocyte Antigen-B (HLA-B) is an MHC-I (major histocompatibility complex) heavy chain receptor, composed of HLA-B and beta2-microglobulin (B2M). There are over 200 genes encoding HLA variants and this variability plays a critical role in adaptive immunity. HLA-B has been linked to autoimmune disorders and drug reactions. HLA-B*40:01 has been linked to ankylosing Spondylitis (AS), a chronic inflammatory rheumatic disorder that targets mainly the axial skeleton and sacroiliac joints. It has also been identified as crucial for the onset and development of anti-tumor responses. The understanding of the association between HLA alleles and diseases will allow a refinement of therapeutic strategies.