Virus-based Tools: Lentivirus, AAV und VSV

Virus-based tools can be used to manipulate genetic material and study viral infections. Our partner BPS Bioscience offers infection systems based on lentiviruses, adeno-associated viruses (AAV) or the vesicular stomatitis virus (VSV). Lentiviruses can be used to generate stably transfected cell lines, they have a high infection rate (>90%), provide high levels of subsequent protein expression, and can be used both in vitro and in vivo. Learn more about lentiviruses and potential applications in our blog. AAVs are useful as viral vectors because they can carry a gene of interest up to 5 kb in length. Because they lead to stable gene expression and they can be used safely due to their low immunogenicity, AAVs are the vectors of choice for the development of many gene therapies. The enveloped VSV provides a valuable tool for studying viral entry, replication, and assembly. VSV-G, the glycoprotein of the virus, is responsible for the attachment and entry of VSVs into the host cell and is often used to pseudotype viral vectors.

Virus-based tools can be used to manipulate genetic material and study viral infections. Our partner  BPS Bioscience offers infection systems based on lentiviruses, adeno-associated... read more »
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Virus-based Tools: Lentivirus, AAV und VSV

Virus-based tools can be used to manipulate genetic material and study viral infections. Our partner BPS Bioscience offers infection systems based on lentiviruses, adeno-associated viruses (AAV) or the vesicular stomatitis virus (VSV). Lentiviruses can be used to generate stably transfected cell lines, they have a high infection rate (>90%), provide high levels of subsequent protein expression, and can be used both in vitro and in vivo. Learn more about lentiviruses and potential applications in our blog. AAVs are useful as viral vectors because they can carry a gene of interest up to 5 kb in length. Because they lead to stable gene expression and they can be used safely due to their low immunogenicity, AAVs are the vectors of choice for the development of many gene therapies. The enveloped VSV provides a valuable tool for studying viral entry, replication, and assembly. VSV-G, the glycoprotein of the virus, is responsible for the attachment and entry of VSVs into the host cell and is often used to pseudotype viral vectors.

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DLL3 Lentivirus
DLL3 Lentivirus

Item number: BPS-78909

DLL3 (Delta-like protein 3) Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles ready to transduce nearly all types of mammalian cells, including primary and non-dividing cells. These particles contain human DLL3 (NM_016941.3) driven by a CMV promoter and a puromycin selection...
Keywords: DLL3, Delta3, Delta-like protein 3, Drosophila Delta homolog 3,
Application: Protein expression, cell pool / stable cell line generation (puromycin selection)
Species reactivity: human
1,490.00€ *
Review
CRE/CREB Luciferase Reporter Lentivirus
CRE/CREB Luciferase Reporter Lentivirus

Item number: BPS-79580

The main role of the cAMP response element, or CRE, is mediating the effects of Protein Kinase A (PKA) by way of transcription. Upon phosphorylation, CREB forms a functionally active dimer that binds the CRE element within the promoters of target genes and activates transcription. CRE is at the focus of many...
Keywords: CREB1, CREB-1, cAMP-responsive element-binding protein 1, Cyclic AMP-responsive element-binding protein 1
Application: Activator / inhibitor screening
Species reactivity: human
1,307.00€ *
Review
SBE Luciferase Reporter Lentivirus (TGFbeta/SMAD Pathway)
SBE Luciferase Reporter Lentivirus (TGFbeta/SMAD Pathway)

Item number: BPS-79806

The SBE Luciferase Reporter Lentivirus (TGFbeta/SMAD signaling pathway) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene...
Keywords: SBE reporter, TGFbeta reporter, TGFbeta reporter, SMAD reporter,
Application: TGFbeta/SMAD signaling pathway activator / inhibitor screening, stable cell line generation
Species reactivity: human
1,373.00€ *
Review
AP1 Luciferase Reporter Lentivirus (JNK Signaling Pathway)
AP1 Luciferase Reporter Lentivirus (JNK Signaling Pathway)

Item number: BPS-79823

The stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) family of proteins includes mitogen-activated protein kinases (MAPKs) that are activated by stress, inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and morphogenesis. Upon activation of the SAPK/JNK pathway, MAP...
Keywords: JNK reporter, AP-1 cell line, activator protein-1 lentivirus,
Application: JNK signaling pathway activator / inhibitor screening, stable cell line generation
Species reactivity: human
1,248.00€ *
Review
STAT3 Luciferase Reporter Lentivirus
STAT3 Luciferase Reporter Lentivirus

Item number: BPS-79744

The STAT3 Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of...
Keywords: Acute-Phase Response Factor, APRF, ADMIO1, HIES,
Application: Activator / inhibitor screening, stable luciferase reporter cell line generation
Species reactivity: human
1,351.00€ *
Review
IL-8 Promoter Luciferase Reporter Lentivirus
IL-8 Promoter Luciferase Reporter Lentivirus

Item number: BPS-79827

The IL-8 Promoter Luciferase Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by the human IL-8...
Keywords: IL-8 Promoter reporter, lentivirus reporter, HIV reporter, VSV-G reporter,
Application: Activator / inhibitor screening, IL-8 promoter luciferase reporter stable cell line generation
Species reactivity: human
1,248.00€ *
Review
FcER1G Lentivirus
FcER1G Lentivirus

Item number: BPS-79878

FcER1G is an adapter protein containing an immunoreceptor tyrosine-based activation motif (ITAM) that transduces activation signals from various immunoreceptors. As a component of the high-affinity immunoglobulin E (IgE) receptor, FcER1G mediates allergic inflammatory signaling in mast cells. FcER1G is also a...
Keywords: FCER1G, FcRgamma, FceRI gamma, Fc-epsilon RI-gamma, Fc receptor gamma-chain, IgE Fc receptor subunit gamma, High affinity...
Application: Transient FcER1G expression, stable cell line generation
Species reactivity: human
1,248.00€ *
Review
Firefly Luciferase Lentivirus (UbC Promoter)
Firefly Luciferase Lentivirus (UbC Promoter)

Item number: BPS-79880

The Firefly Luciferase Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene driven by a UbC (ubiquitin C) promoter. The...
Keywords: UbC lentivirus,
Application: Transduction positive control, transduction optimization, stable cell line generation
Species reactivity: common eastern firefly
1,248.00€ *
Review
Renilla Luciferase (Rluc) Lentivirus (G418)
Renilla Luciferase (Rluc) Lentivirus (G418)

Item number: BPS-79565-G

The Renilla Luciferase Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles constitutively express Renilla luciferase under a CMV promoter.
Keywords: Renilla-type luciferase, Coelenterazine h 2-monooxygenase, Renilla-luciferin 2-monooxygenase,
Application: Internal control, stable cell line generation
1,285.00€ *
Review
NFAT eGFP Reporter Lentivirus
NFAT eGFP Reporter Lentivirus

Item number: BPS-79922

The NFAT eGFP Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an enhanced GFP gene driven by the NFAT response element located...
Application: Activator / inhibitor screening, stable cell line generation
1,424.00€ *
Review
COVID-19 Research
TMPRSS2 Lentivirus
TMPRSS2 Lentivirus

Item number: BPS-78011-1

Human transmembrane serine protease 2 (TMPRSS2) is an enzyme primarily expressed by endothelial cells across the respiratory and digestive tracts. It is involved in viral entry and spread of coronaviruses including SARS-CoV-2, the virus that causes COVID19. TMPRSS2 primes the spike protein by cleaving at the S1/S2...
Keywords: PRSS10,
Application: Transient TMPRSS2 expression in target cells, stable TMPRSS2 over-expressing cell line generation
Species reactivity: human
From 1,248.00€ *
Review
Negative Control eGFP Reporter Lentivirus
Negative Control eGFP Reporter Lentivirus

Item number: BPS-79927

The Negative Control eGFP Reporter Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to infect almost all types of mammalian cells, including primary and non-dividing cells. The particles contain an enhanced Green Fluorescent Protein (eGFP) gene under the...
Keywords: enhanced GFP reporter, negative control pseudovirion, eGFP pseudovirus negative control,
Application: eGFP reporter activity background determination, establishing lentivirus reporter system specificity
Species reactivity: mammalian
1,307.00€ *
Review
3 from 29 pages