Endoplasmic Reticulum (ER) eGFP Lentivirus

Endoplasmic Reticulum (ER) eGFP Lentivirus are replication incompetent, HIV based, VSV-G pseudotyped lentiviral particles that are ready to transduce almost all types of mammalian cells, including primary and non-dividing cells. These particles contain eGFP (enhanced green fluorescent protein), with an ER-targeting and retrieval sequences at both the N and C-terminus of eGFP, respectively, under the control of an EF1a promoter. The lentiviruses also transduce a puromycin selection marker (Figure 1). eGFP expression and transduction efficiency can easily be verified and optimized via fluorescence microscopy or flow cytometry.