Anti-CD20 CAR Lentivirus (Clone 3H7-CD8-41BB-CD3zeta)

The Anti-CD20 CAR Lentivirus (Clone 3H7-CD8-41BB-CD3zeta) are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to transduce all types of mammalian cells, including primary and non-dividing cells. These viruses transduce the ScFv portion of anti- CD20 (clone 3H7) linked to a 2nd generation CAR (Chimeric Antigen Receptor), containing the CD8 hinge and transmembrane domains, 4-1BB and CD3zeta signaling domains (Figure 1). These lentiviruses have been validated by flow cytometry (to determine the CAR expression) and in co-culture cytotoxicity assays. A. B. Figure 1: Anti-CD20 CAR Lentivirus (Clone 3H7-CD8-41BB-CD3zeta) construct diagrams. (A) Schematic of the lenti-vector used to generate the anti-CD20 CAR lentivirus. (B) Construct diagram showing components of the anti-CD20 CAR.