WM983BBR Purified RNA

Total RNA was extracted and purified from cells using RNA isolation & purification kit according to manufacturers instructions. The RNA was diluted to 200 ng/µL in RNase free water. Concentration was determined at A260 using nanodrop ND-1000. Total RNA was prepared from cell line WM983BBR. WM983BBR is a human metastatic melanoma cell line that displays a mesenchymal morphology. The WM983BBR cell line contains the BRAF V600E mutation that causes increased signaling via the extracellular signal-regulated MAPK/ERK kinase pathways to enhance proliferation.  WM983BBR is wild type for PTEN, N-ras, c-KIT, and CDK4. This cell line was derived from the same pateint as the cell lines WM983A, WM983B, and WM983C. The WM983BBR cells produce xenograft tumors when injected into immunocompromised mice.