WM873-2 Purified Genomic DNA

Genomic DNA was purified from cells using genomic DNA preparaton kit according to manufacturers instruction. DNA was diluted to 200 ng/µL in TE buffer (0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6). Concentration was determined at A260 using nanodrop ND-1000. DNA was prepared from cell line WM873-2. WM873-2 is a metastatic human melanoma cell line that displays a flat mesenchymal morphology. This cell line expresses PTEN loss of function including hemizygous deletion. WM873-2 cells match with WM858 and WM873-1. These cells produce xenograft tumors when injected into immunocompromised mice.