WM853-2 Purified RNA

Total RNA was extracted and purified from cells using RNA isolation & purification kit according to manufacturers instructions. The RNA was diluted to 200 ng/µL in RNase free water. Concentration was determined at A260 using nanodrop ND-1000. Total RNA was prepared from cell line WM853-2. WM853-2 is a tumorigenic (VGP) primary melanoma cell line with competence for metastasis. This cell line was established from a back metastasis site in a 71-year-old male. This cell line features the specific V600E (Val600Glu) mutation at codon 600 in the BRAF gene. This mutation causes constitutively active kinase activity and activation of MEK and ERK signaling pathway. This cell line expresses wild type PTEN, N-RAS, c-KIT, and CDK4 genes. WM853-2 cells produce xenograft tumors when injected into immunocompromised mice.