WM3618F Purified Genomic DNA

Genomic DNA was purified from cells using genomic DNA preparaton kit according to manufacturers instruction. DNA was diluted to 200 ng/µL in TE buffer (0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6). Concentration was determined at A260 using nanodrop ND-1000. DNA was prepared from cell line WM3618. WM3618F is a human metastatic uveal melanoma cell line displaying melanocytic morphology. These cells require 10% FBS to adhere to culture flask. This cell line was established from a lymph node metastases arising from uveal melanoma of a patient. Uveal melanoma is a rare type of cancer of an eye and can arise in the anterior (iris) or the posterior (ciliary body or choroid) uveal tract. Most uveal tract melanomas originate in the choroid. The ciliary body is less commonly a site of origin, and the iris is the least common. WM3618F cells match with WM3772F. These cells produce xenograft tumors when injected into immunocompromised mice.