WM3246 Purified Genomic DNA

Genomic DNA was purified from cells using genomic DNA preparaton kit according to manufacturers instruction. DNA was diluted to 200 ng/µL in TE buffer (0.01 M Tris Chloride, 0.001 M EDTA, pH 7.6). Concentration was determined at A260 using nanodrop ND-1000. DNA was prepared from cell line WM3246. WM3246 is a metastatic human melanoma cell line that was established from an adrenal gland metastatic site of a female patient. This cell line expresses mutated PTEN gene and is wild type for BRAF, N-RAS, c-KIT and CDK4. WM3246 cells produce xenograft tumors when injected into immunocompromised mice.