Phosphotyrosine Control, EGF-Stimulated A431 Cell, Lysate

Applications: , Positive control for anti-phosphotyrosine antibodies. Suitable for use in Western Blot, Immunoprecipitation. Recommended Dilution: Western Blot: Detects the phosphorylated EGF receptor (170kD) and other tyrosine phosphorylated proteins in 20ug of the preparation. Add an equal volume of Laemmli reducing sample buffer to 5-25ul of lysate a,nd boil for 5 min to reduce the preparation. Immunoprecipitation Positive Control: This preparation may also be used for immunoprecipitation with anti-phosphotyrosine agarose. , Storage and Stability:, Store at -20ºC Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.