A431 Pervanadate Control Lysate

Pervanadate is a protein tyrosine phosphatase inhibitor that is commonly used to increase tyrosine phosphorylation in cells. When cells are treated with pervanadate for 30 minutes they undergo significant tyrosine phosphorylation, as shown by Western blotting using anti-Phosphotyrosine. Confluent cultures of A431 cells were serum starved overnight. Cells were then either left untreated (A0001-52B) or treated with pervanadate (1mM) for 30 minutes at 37°C (A0001-52E). Cells were lysed in 1% SDS, 1mM sodium ortho-vanadate, 10mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer. Applications: SSuitable for use in Western Blot Other applications not tested. Recommended Dilutions: Western Blot: 20ul/lane Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.