Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such... read more »
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Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

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HL-60 Whole Cell Lysate
HL-60 Whole Cell Lysate

Item number: W09-001-GK8

HL-60 cells were grown in Ioscove's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
220.00€ *
Review
HL-60 Cell Nuclear Extract
HL-60 Cell Nuclear Extract

Item number: W09-001-GK9

HL-60 cells were grown in Ioscove's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
325.00€ *
Review
U-937 Whole Cell Lysate
U-937 Whole Cell Lysate

Item number: W09-001-GL1

U-937 cells were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
220.00€ *
Review
U-937 Cell Nuclear Extract
U-937 Cell Nuclear Extract

Item number: W09-001-GL2

U-937 cells were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
325.00€ *
Review
HT-1080 Cell Nuclear Extract
HT-1080 Cell Nuclear Extract

Item number: W09-001-GL4

HT-1080 cells were grown in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01%...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
325.00€ *
Review
A-172 Whole Cell Lysate
A-172 Whole Cell Lysate

Item number: W09-001-GL5

A-172 cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
220.00€ *
Review
A-172 Cell Nuclear Extract
A-172 Cell Nuclear Extract

Item number: W09-001-GL6

A-172 cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
325.00€ *
Review
PANC-1 Cell Nuclear Extract
PANC-1 Cell Nuclear Extract

Item number: W09-001-GM3

PANC-1 cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
325.00€ *
Review
HCT-116 Whole Cell Lysate
HCT-116 Whole Cell Lysate

Item number: W09-001-GM4

HCT-116 cells were grown in McCoy's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
220.00€ *
Review
HCT-116 Cell Nuclear Extract
HCT-116 Cell Nuclear Extract

Item number: W09-001-GM5

HCT-116 cells were grown in McCoy's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
325.00€ *
Review
Normal Mouse Spleen Whole Cell Lysate
Normal Mouse Spleen Whole Cell Lysate

Item number: W10-000-T029

1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer
Application: WB
220.00€ *
Review
NIH/3T3 Cell Nuclear Extract
NIH/3T3 Cell Nuclear Extract

Item number: W10-001-A74

1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer. Ready-to-use nuclear extract especially prepared as positive control for separation by SDS-PAGE and subsequent western blot analysis.
Application: WB, Posive control
325.00€ *
Review
8 from 12 pages