Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such... read more »
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Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

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Hep-G2 Lysate
Hep-G2 Lysate

Item number: W09-001-GJ5

Hep-G2 cells were grown in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v)...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
220.00€ *
Review
HepG2 Nuclear Cell Lysate
HepG2 Nuclear Cell Lysate

Item number: W09-001-GJ6

Hep-G2 cells were grown in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v)...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
325.00€ *
Review
K-562 Whole Cell Lysate
K-562 Whole Cell Lysate

Item number: W09-001-GJ7

K-562 cells were grown in Ioscove's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
220.00€ *
Review
K-562 Cell Nuclear Extract
K-562 Cell Nuclear Extract

Item number: W09-001-GJ8

K-562 cells were grown in Ioscove's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
325.00€ *
Review
LNCaP Whole Cell Lysate
LNCaP Whole Cell Lysate

Item number: W09-001-GJ9

LNCap cells were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
220.00€ *
Review
LNCaP Cell Nuclear Extract
LNCaP Cell Nuclear Extract

Item number: W09-001-GK1

LNCap cells were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
325.00€ *
Review
MOLT-4 Whole Cell Lysate
MOLT-4 Whole Cell Lysate

Item number: W09-001-GK2

MOLT-4 cells were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IHC, IP, WB
Host: Human
Species reactivity: human
220.00€ *
Review
MOLT-4 Cell Nuclear Extract
MOLT-4 Cell Nuclear Extract

Item number: W09-001-GK3

MOLT-4 cells were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
325.00€ *
Review
Ramos Whole Cell Lysate
Ramos Whole Cell Lysate

Item number: W09-001-GK4

Ramos cells were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
220.00€ *
Review
Ramos Cell Nuclear Extract
Ramos Cell Nuclear Extract

Item number: W09-001-GK5

Ramos cells were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
325.00€ *
Review
MDA-MB-231 Whole Cell Lysate
MDA-MB-231 Whole Cell Lysate

Item number: W09-001-GK6

MDA-MB-231 cells were grown in Dubelcco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
220.00€ *
Review
MDA-MB-231 Cell Nuclear Extract
MDA-MB-231 Cell Nuclear Extract

Item number: W09-001-GK7

MDA-MB-231 cells were grown in Dubelcco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
325.00€ *
Review
7 from 12 pages