Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such... read more »
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Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

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Normal Rat Kidney Whole Cell Lysate
Normal Rat Kidney Whole Cell Lysate

Item number: W12-000-T090

1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer
Application: WB
220.00€ *
Review
MAP Kinase, p38 Control Cell Extracts (MAPK, Erk2, Extracellular Signal Regulated Kinase, RK, CSBP,
MAP Kinase, p38 Control Cell Extracts (MAPK, Erk2,...

Item number: M2352-01C.10

P38 MAP kinase (MAPK), also called RK (3) or CSBP (2), is the mammalian homologue of the yeast HOG kinase and participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, alpha, beta, gamma and delta, have been identified so far. Like the SAPK/JNK...
Application: WB
517.00€ *
Review
HT-29 Lystate
HT-29 Lystate

Item number: W09-001-BS0

HT-29 cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: IP, WB
Host: Human
Species reactivity: human
220.00€ *
Review
HT-1080 Whole Cell Lysate
HT-1080 Whole Cell Lysate

Item number: W09-001-GL3

HT-1080 cells were grown in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01%...
Application: ChIP, FC, IP, WB
Host: Human
Species reactivity: human
220.00€ *
Review
PC-12 Whole Cell Lysate
PC-12 Whole Cell Lysate

Item number: W12-001-GL9

PC-12 cells were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to...
Application: ChIP, IP, WB
Host: Rat
Species reactivity: rat
220.00€ *
Review
Helicobacter pylori, Lysate
Helicobacter pylori, Lysate

Item number: H1840-04.1

Helicobacter pylori is a spiral-shaped, Gram-negative rod approximately 0.5x3.0um in size. The catalase-positive organism has 4-6 sheathed flagella attached to one pole which allow for motility. Helicobacter pylori lives in the stomach and duodenum (section of intestine just below stomach). It has a unique way of...
Application: ELISA
1,039.00€ *
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Seminal Vesicles, Ram, Microsomal Fraction
Seminal Vesicles, Ram, Microsomal Fraction

Item number: S0680-05.5

Microsomal fraction of ram seminal vesicles., Each vial should be sufficient for ~100 oxygraph assays, Applications: Suitable for use as a source for isolation of several eicosanoid metabolizing enzymes. Useful for enzyme purification or by addition of inhibitors, as a crude source of specific enzyme activities....
644.00€ *
Review
Normal Chicken Spinal Cord Whole Cell Lysate
Normal Chicken Spinal Cord Whole Cell Lysate

Item number: W03-001-T296

1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer
Application: WB
325.00€ *
Review
A431Cell Nuclear Extract
A431Cell Nuclear Extract

Item number: W09-001-A60

Ready-to-use whole cell lysate 1.0 mg/ml by Lowry assay in 1X SDS-PAGE sample buffer.
Application: WB, Positive control
295.00€ *
Review
HeLa Cell Nuclear Extract - Nocodozole Stimulated (1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Bu
HeLa Cell Nuclear Extract - Nocodozole Stimulated (1.0...

Item number: W09-001-A84

Ready to use. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added).
Application: WB, Positive control
298.00€ *
Review
MCF-7 Cell Nuclear Extract (1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer)
MCF-7 Cell Nuclear Extract (1.0 mg/ml by Lowry assay in...

Item number: W09-001-A85

Ready to use. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added).
Application: WB, Positive control
295.00€ *
Review
HeLa Cell Nuclear Extract - TNFa Stimulated (1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer)
HeLa Cell Nuclear Extract - TNFa Stimulated (1.0 mg/ml by...

Item number: W09-001-A86

Ready to use. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added).
Application: WB, Positive control
298.00€ *
Review
6 from 12 pages