Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such... read more »
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Lysates

A cell lysate is produced by enzymatic, osmotic or mechanical disruption of the plasma membrane of a population of cells. Cell lysis is utilized during the purification of cellular components such as DNA, RNA, proteins or whole organelles. A lysate of cells over-expressing a certain protein is commonly used as a positive control in western blots and ELISAs to evaluate the target specificity of an antibody.

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Mouse Lung, Adult, Whole Cell Lysate
Mouse Lung, Adult, Whole Cell Lysate

Item number: W10-000-MQ1

Ready-to-use whole cell lysates produced by Rockland Immunochemicals are derived from cell lines or tissues using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels and immunoblot...
Application: WB
Host: Mouse
Species reactivity: mouse
220.00€ *
Review
Rat brain, post natal, Whole Cell Lysate
Rat brain, post natal, Whole Cell Lysate

Item number: W12-001-MQ6

Multi-purpose Whole Cell Lysates produced by Rockland Immunochemicals are derived from tissues and cell lines using highly refined extraction protocols to ensure exceptionally high quality, protein integrity and lot-to-lot reproducibility. All extracts are tested by SDS-PAGE using 4-20% gradient gels. Tissues were...
Application: ELISA, IP, WB
Host: Rat
Species reactivity: rat
325.00€ *
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U-87 MG Whole Cell Lysate
U-87 MG Whole Cell Lysate

Item number: W09-001-GX2

U-87 MG cells were grown in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01%...
Application: WB
Host: Human
Species reactivity: human
220.00€ *
Review
U-251 Whole Cell Lysate
U-251 Whole Cell Lysate

Item number: W09-001-GY4

U-251 cells were grown in Eagle's Minimum Essential Medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v)...
Application: WB
Host: Human
Species reactivity: human
220.00€ *
Review
U-138 MG Whole Cell Lysate
U-138 MG Whole Cell Lysate

Item number: W09-001-MP5

The U-138 MG cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium...
Application: WB
Host: Human
Species reactivity: human
220.00€ *
Review
Spleen Extract, Bovine, Purified
Spleen Extract, Bovine, Purified

Item number: S5550-05A.1

Activities include Ro (SS-A), La (SS-B), Sm, and RNP, Reconstitution: Reconstitute using 1ml of ddH2O. Vortexing will speed dissolution. Applications: Optimal for autoantigen activity in immunodiffusion assays. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the...
627.00€ *
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Transcription Factor p53 Positive Control
Transcription Factor p53 Positive Control

Item number: Cay600022-1

Formulation: 150 µl of Nutlin-3-stimulated MCF-7 nuclear extract. Origin: Animal/Bovine.
Application: Control
119.00€ *
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Normal Mouse Brain Whole Cell Lysate in 1 x SDS-PAGE Sample Buffer
Normal Mouse Brain Whole Cell Lysate in 1 x SDS-PAGE...

Item number: W10-000-T004

Ready-to-use
Application: WB, Positive control
220.00€ *
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Beef Extract (Peptic Digest of Animal Tissue)
Beef Extract (Peptic Digest of Animal Tissue)

Item number: B1000.500

Beef Extract is a desiccated powder used as a substitute for infusions of meat for culture media. Appearance: Light tan powder, Solubility: Gold, clear, complete, Total Nitrogen: >11%, Loss on Drying: <5%, Ash: <19%, pH (2%): 6.5-7.5, Standard Plate Count: <1000cfu/g, Yeast and Mold: <100cfu/g, Coliforms: <10cfu/g,...
From 167.00€ *
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PANC-1 Whole Cell Lysate
PANC-1 Whole Cell Lysate

Item number: W09-001-GM2

PANC-1 cells were grown in Dulbecco's medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide...
Application: ChIP, IP, WB
Host: Human
Species reactivity: human
220.00€ *
Review
Normal Mouse Kidney Whole Cell Lysate
Normal Mouse Kidney Whole Cell Lysate

Item number: W10-000-T017

1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer
Application: WB
220.00€ *
Review
Normal Rat Brain Whole Cell Lysate
Normal Rat Brain Whole Cell Lysate

Item number: W12-000-T077

1.0 mg/ml by Lowry assay in 1X SDS-PAGE Sample Buffer
Application: WB
220.00€ *
Review
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