T47D Cells

Categories: Breast cancer cell lines Description: The T47D cell line, originating from the pleural effusion of an infiltrating ductal carcinoma of the breast, has become a critical resource in breast cancer research. T-47D cells are unique in the realm of cancer research for their hormonal expression profile, particularly for carrying receptors for 17 beta estradiol, various other steroids, and calcitonin. Additionally, T47D cells express the WNT7B oncogene. T47D cells are notable for their progesterone receptor expression not being regulated by estradiol, despite the hormone's abundance within the cells, setting them apart from MCF7 cells, which are widely recognized for their estrogen receptor positivity and are frequently used to explore estrogen's role in tumor proliferation and response to therapies. The utility of the T47D cell line extends to the formation of xenografts in immunodeficient mice, which are valuable for drug testing, observing receptor status changes, and studying angiogenesis. Furthermore, the T-47D cell line is a resource for cancer gene studies, providing insights into the genomic and proteomic landscape that drives breast cancer. By facilitating a deeper understanding of the proteomic and transcriptomic profiles of breast cancer, the t47d breast cancer cell line aids in the identification of new breast cancer cell phenotypes and the development of targeted therapies. T47D cells have been instrumental in studying the effects of hormones like progesterone on breast cancer, offering insights into transcriptional regulation, drug resistance, and the development of xenograft models for therapeutic testing. Organism: Human Tissue: Breast Disease: Invasive ductal carcinoma Metastatic Site: Pleural effusion Synonyms: T-47-D, T47-D, T47D:A, T47D Age: 54 years Gender: Female Ethnicity: Caucasian Morphology: Epithelial-like Growth Properties: Monolayer, adherent Citation: T47D (Cytion catalog number 300353) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_0553 Receptors Expressed: Estradiol, steroids, calcitonin, androgen, progesterone, glucocorticoid, prolactin, estrogen Isoenzymes: G6PD, B, PGM1, 1, PGM3, 1, ES-D, 2, Ak-1, 1, GLO-1, 1-2 Oncogenes: wnt3 +, wnt7h +, wnt7b+ Tumorigenic: Yes, in nude mice Mutational Profile: TP53 mut Karyotype: Mode = 66, dicentric and extra long submetacentric chromosomes Culture Medium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) Supplements: Supplement the medium with 10% FBS, 10 microgram/ml HREC insulin Dissociation Reagent: Accutase Subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. Seeding Density: 1 x 104 cells/cm2 Fluid Renewal: 2 to 3 times per week Post Thaw Recovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820700a Required Product 3: 860015.0 Required Product 4: 830100.0