SH-SY5Y Cells

Categories: Neuroblastoma cell lines Description: SH-SY5Y cells, a subclone derived from the neuroblastoma cancer cell line SK-N-SH, are a valuable cell model for neurodegenerative disorders such as Parkinson's and Alzheimer's disease. The SK-N-SH cell line was established in 1970 from a biopsy of a metastatic bone tumor from a 4-year-old cancer patient. The human SH-SY5Y cell line offers a unique cell source for functional studies in neurobiology and neurodegenerative disease research. SH-SY5Y cells grow both adherently and in suspension, forming clusters during division that differ significantly from the morphology of differentiated cells. These undifferentiated cells, before undergoing neuronal differentiation, serve as an essential foundation for neuroscientific studies. The neuronal differentiation of SH-SY5Y cells, which transforms them into neuronal cell models resembling various functional neurons, is achieved through biochemical interconversion processes involving gradual serum deprivation, retinoic acid, neurotrophic factors like brain-derived neurotrophic factor, and extracellular matrix proteins. This differentiation is crucial for studying neuronal markers and conducting neurotoxicology research, especially concerning the impact of organic pollutants on human neuron-like cells. The neurobiology of SH-SY5Y neuroblastoma cells, primarily known for their dopaminergic characteristics, can be explored for cholinergic properties under specific differentiation conditions. While these cells may express acetylcholinesterase, indicative of some cholinergic activity, their utility in studying cholinergic neurotransmission is less pronounced compared to their role in dopaminergic system studies. As a neurotoxicological model, the SH-SY5Y neuroblastoma cell line is instrumental in examining the effects of compounds on acetylcholinesterase and butyryl cholinesterase activities, essential for neurotoxicology studies. The sy5y line's contribution to understanding the biochemical pathways involved in neurodegenerative diseases, coupled with its role in the functional studies of dopaminergic and cholinergic systems, underscores its value in neuroscience research. Organism: Human Tissue: Bone Marrow Disease: Neuroblastoma Metastatic Site: Bone marrow Synonyms: SH-Sy5y, SHSY5Y, SHSY-5Y, SK-SH-SY5Y, SY5Y, SH-SY5Y Parental Age: 4 years Gender: Female Morphology: The cells grow as clusters of neuroblastic cells with multiple, short, fine cell processes (neurites). Cells will aggregate, form clumps and float. A confluent monolayer is not formed. Cell Type: Neuroblast Growth Properties: Loosely adherent and form clumps at high cell density Citation: SH-SY5Y (Cytion catalog number 300154) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_0019 Tumorigenic: Forms tumors in nude mice within approx. 3-4 weeks. Karyotype: The cytogenetic landscape of SH-SY5Y cells is marked by complex chromosomal aberrations, notably featuring a modal chromosome number of 47, including trisomy of 1q due to a distinctive insertion in chromosome 1. This genetic backdrop is crucial for understanding the cellular biology and oncogenic potential of SH-SY5Y cells, making them a versatile model in neuroscientific research, particularly in the realms of neurodevelopment, neurotoxicity, and neurodegenerative disease studies. Culture Medium: Please mix EMEM and Ham's F12 in a 50:50 ratio (Cytion article numbers 820100a and 820600a) Supplements: Supplement the medium with 15% FBS Dissociation Reagent: Accutase Subculturing: These cells grow as a mixture of floating and adherent cells. Remove the medium with the floating cells, and recover the cells by centrifugation. Rinse the adherent cells using PBS without calcium and magnesium (3-5 ml PBS for T25, 5-10ml for T75 cell culture flasks). Add Accutase (1-2ml per T25, 2.5ml per T75 cell culture flask), the cell sheet must be covered completely. Incubate at 37 degree Celsius for 10 minutes. Combine with the floating cells recovered above. Carefully resuspend the cells, the addition of medium is optional but not necessary, and dispense into new flasks which contain fresh medium. Seeding Density: Seeding density after thawing 6x104 cells/cm2, seed into 1x T25 cell culture flask. The cells will become 80-90% confluent within 1-2 weeks. Once the cells proliferate vigorously, seed out the cells at a density of 1 - 2 x104 cells/cm2. Fluid Renewal: 1 to 2 times per week Freeze Medium: As a cryopreservation medium, use 50% basal medium + 40% FBS + 10% DMSO, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820100a Required Product 2: 820600a Required Product 3: 860015.0 Required Product 4: 830100.0