NF-kappaB Luciferase-eGFP Reporter HEK293 Cell Line

NF-kappaB Luciferase-eGFP Reporter HEK293 Cell Line is a HEK293 cell line designed to monitor nuclear factor Kappa B (NF-kappaB) activity. It contains a firefly luciferase-eGFP (enhanced green fluorescent protein) reporter driven by multiple copies of the NF-kappaB response element located upstream of the minimal TATA promoter. After activation by pro-inflammatory cytokines or agonists of the lymphokine receptors, endogenous NF-kappaB transcription factors bind to the DNA response elements, inducing transcription of the luciferase-eGFP reporter. The cell line has been functionally validated in response to human TNF-alpha.