NCI-H146 Cells

Categories: Lung cancer cell lines Description: The NCI-H146 cell line was derived by A.F. Gazdar and associates in 1979 from the pleural fluid of a patient with small cell cancer of the lung. The bone marrow specimen was taken prior to therapy. Organism: Human Tissue: Lung Disease: Small cell carcinoma Metastatic Site: Bone marrow Synonyms: H146, H-146, NCIH146 Age: 59 years Gender: Male Ethnicity: Caucasian Morphology: Epithelial-like Growth Properties: Aggregates in suspension Citation: NCI-H146 (Cytion catalog number 300182) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_1473 Receptors Expressed: insulin-like growth factor II receptor (IGF II) Protein Expression: The cells stain positively for vimentin and keratin, but are negative for neurofilament triplet protein. Antigen Expression: The line expresses elevated levels of four biochemical markers: neuron specific enolase, brain isoenzyme of creatine kinase, L-DOPA decarboxylase and bombesin-like immunoreactivity Isoenzymes: G6PD, B, PGM1, 1-2, PGM3, 1-2, ES-D, 1, Me-2, 2, AK-1, 1, GLO-1, 1, Phenotype Frequency Product = 0.0009 Tumorigenic: Forms transplantable tumors in nude mice which histologically resemble tumor cells from the original biopsy specimen Products: The cells produce relatively high amounts of c-myc mRNA, but c-myc DNA sequences are not amplified. The cells do not express vasopressin, oxytocin or gastrin releasing peptide. Ploidy Status: Aneuploid Msi Status: Stable (MSS) Karyotype: This is a near triploid human cell line. The modal chromosome number is 68, but cells with 66, 70 and 71 chromosomes also occurred frequently. The x chromosomes were paired, and no Y chromosome was detected in QM stained preparations. Culture Medium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) Supplements: Supplement the medium with 10% heat-inactivated FBS Subculturing: The cells should be subcultured by transferring part of the suspension into fresh new cell culture flasks prefilled with fresh medium. Alternatively, the clusters may be collected by centrifugation and resuspended in fresh medium. Seeding Density: 1 to 2 x 105 cells/ml Fluid Renewal: 2 to 3 times per week Post Thaw Recovery: After thawing allow the cells to recover from the freezing process for at least 24 to 48 hours. Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820700a Required Product 3: 860015.0 Required Product 4: 830100.0