NB-4 Cells

Categories: Leukemia cell lines Description: NB-4 cells are a human acute promyelocytic leukemia (APL) cell line established from the bone marrow of a patient experiencing the second relapse of acute promyelocytic leukemia. This cell line is characterized by the presence of the t(15,17) chromosomal translocation, which results in the PML-RARalpha fusion gene, a hallmark of APL. The NB4 cell line serves as a pivotal model for studying the pathogenesis of APL and the mechanisms of action of therapeutic agents inducing differentiation such as retinoic acid (ATRA) and arsenic trioxide (ATO). As a promyelocytic leukemia cell line, NB-4 cells exhibit an aberrant pattern of differentiation that is characteristic of APL. This aberrancy provides a unique window into the cellular mechanisms underlying leukemia progression and the potential for therapeutic intervention. The ability of NB-4 cells to undergo apoptosis, or programmed cell death, upon exposure to certain chemotherapeutic agents or differentiation inducers like retinoic acid, makes them an invaluable tool for studying cell apoptosis in the context of leukemia. The NB-4 cell line also demonstrates bilineage potential, highlighting its ability to differentiate along multiple hematopoietic lineages under specific conditions. In conclusion, the NB-4 cell line, with its unique properties and responsiveness to differentiation inducers like retinoic acid, continues to be a pivotal resource for researchers delving into the intricacies of promyelocytic leukemia and the broader field of oncology. Organism: Human Tissue: Bone marrow Disease: Acute promyelocytic leukemia Synonyms: NB4, NB.4 Age: 23 years Gender: Female Ethnicity: Caucasian Morphology: Round cells Cell Type: B lymphocyte Growth Properties: Suspension Citation: NB-4 (Cytion catalog number 300299) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_0005 Antigen Expression: CD4+, CD14-, CD36- Reverse Transcriptase: Negative Karyotype: t(15,17) (q22,q11-12) translocation Culture Medium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) Supplements: Supplement the medium with 10% FBS Doubling Time: 35 to 40 hours Subculturing: Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 105 cells/ml and keep the cell concentration within the range of 3 x 105 to 1 x 106 cells/ml for optimal growth. Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820700a Required Product 3: 860015.0 Required Product 4: 830100.0