MV4-11 Cells

Categories: Leukemia cell lines Description: The MV-4-11 cell line, isolated from the blast cells of a child with biphenotypic B-myelomonocytic leukemia, serves as a critical resource in the study of acute leukemias, particularly acute myeloid leukemia (AML). MV4-11 cells are characterized by their high proliferation rate and the presence of certain genetic abnormalities. A translocation between chromosomes 4 and 11 leads to the creation of the MLL-AF4 fusion gene, which plays a crucial role in leukemogenesis and contributes to the aggressive nature of leukemia. The presence of the MLL-AF4 fusion gene makes these cells particularly relevant for understanding the molecular mechanisms underlying leukemogenesis and studies on targeted therapies that aim to disrupt the function of this oncogenic fusion protein. Additionally, MV4-11 cells can be used to study the biology of leukemia stem cells, drug resistance mechanisms, and the role of the bone marrow microenvironment in leukemia progression. The cell line is further instrumental in metabolomics and transcriptomic profiles research, providing a comprehensive understanding of the metabolic alterations and redox adaptation in leukemia. The ability of MV-4-11 cells to respond to various cancer research chemicals, including inhibitors like venetoclax, and their role in studying resistant cells. In conclusion, the MV-4-11 cell line is a crucial tool in leukemia research, offering a versatile platform for investigating the complex biology of acute myeloid leukemia, testing the efficacy of therapeutic agents, and exploring the potential of targeted treatments in overcoming drug resistance. Organism: Human Tissue: Blood Disease: Acute monocytic leukemia Synonyms: MV-4-11, MV-4:11, MV4:11, MV 4,11, MV4,11, MV411, MV(4,11), Age: 10 years Gender: Male Ethnicity: Caucasian Morphology: Round cells Cell Type: Myelomonocytic, biphenotypic Growth Properties: Suspension Citation: MV4-11 (Cytion catalog number 300295) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_0064 Antigen Expression: CD4 (40-96%), CD10 (4-11%), CD15 (96-99%) Mutational Profile: FLT3mut (a FLT3 internal tandem duplication was verified by PCR) Karyotype: 48, xY, t(4,11)(q21,q23), +8, +19 Culture Medium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) Supplements: Supplement the medium with 10% FBS Subculturing: Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 105 cells/ml and keep the cell concentration within the range of 3 x 105 to 1 x 106 cells/ml for optimal growth. Seeding Density: 5 x 105 cells/mL Post Thaw Recovery: Please allow the cells to recover from the freezing process for at least 48 hours. Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820700a Required Product 3: 860015.0 Required Product 4: 830100.0