MRC2 CHO Cell Line (Low Expression)

MRC2 CHO Cell Line (Low or High Expression) is a CHO-K1 cell line that expresses human MRC2 (mannose receptor C-type 2) protein (accession number NM_006039.5) under the control of the cytomegalovirus (CMV) promoter. This cell line was generated by lentiviral transduction followed by puromycin selection and limiting dilution. Individual clones were screened based on MRC2 expression levels to obtain this cell line. This cell line has been validated by flow cytometry. MRC2, also known as mannose receptor C-type 2, uPARAP or Endo180, is a transmembrane receptor characterized by a cysteine-rich N-terminal domain, a fibronectin type II domain, 8 C-type lectin-like domains, and a transmembrane region. MRC2 functions as a recycling endocytic receptor that facilitates collagen uptake and intracellular collagen degradation. It is expressed throughout embryonic development and in adult tissue fibroblasts and macrophages where it plays a role in tissue remodeling and ECM (extracellular matrix) maintenance. MRC2 is upregulated in a variety of cancers including sarcoma and leukemia, as well as in cancer-associated fibroblasts, where it contributes to ECM remodeling and tumor metastasis, making it a promising therapeutic target. Current therapies being developed are focusing primarily on antibody-drug conjugates (ADCs) that leverage MRC2's efficient trafficking as a recycling endocytic receptor to shuttle/internalize cytotoxic drugs into tumors and malignant cells.