MPC5 Cells

Categories: Mouse cell lines Description: The MPC5 cell line is a conditionally immortalized mouse podocyte model developed to enable studies of podocyte differentiation and function under controlled conditions. This cell line was derived from podocytes isolated from the glomeruli of transgenic mice expressing a temperature-sensitive SV40 large T antigen under the control of an interferon-inducible promoter. Under permissive conditions (33°C with interferon), the MPC5 cells proliferate as undifferentiated, Epithelial-like cells. However, when cultured under non-permissive conditions (37°C without interferon), they undergo growth arrest and differentiation, acquiring features of mature podocytes, such as the formation of arborized processes and expression of podocyte-specific markers like synaptopodin and WT-1. MPC5 cells have proven valuable for in vitro studies of podocyte biology, including cytoskeletal reorganization, differentiation processes, and functional responses to stimuli. During differentiation, these cells exhibit rearrangements of actin and microtubular networks that mimic in vivo podocyte morphology, forming complex foot processes critical for the glomerular filtration barrier. Electrophysiological studies have shown that differentiated MPC5 cells respond to bradykinin via the B2 receptor, indicating functional signaling pathways relevant to podocyte physiology. This cell line has also been instrumental in understanding high glucose-induced podocyte injury, where it has been shown that oxidative stress, cytoskeletal damage, and apoptosis occur, paralleling the pathogenesis of diabetic nephropathy. Recent research highlights the role of molecular regulators, such as miR-204-3p, in modulating high glucose-induced dysfunction in MPC5 cells. This microRNA targets the bradykinin B2 receptor, mitigating apoptotic and oxidative damage pathways activated in hyperglycemic conditions. Such findings underscore the utility of MPC5 cells as a robust model for exploring podocyte-related diseases and testing therapeutic interventions. Organism: Mouse Tissue: Kidney Disease: Normal Synonyms: MPC-5, Mouse Podocyte Clone-5 Breed: (CBA/Ca x C57BL/10)Tg(H2Kb-tsA58) Immortomouse Age: Unspecified Gender: Unspecified Cell Type: Podocyte Growth Properties: Adherent Citation: MPC5 (Cytion catalog number 305481) Biosafety Level: 2 Ncbi_ Taxid: 10090.0 Cellosaurus Accession: CVCL_AS87 Viruses: Transformant: Simian virus 40 (SV40) Culture Medium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) Supplements: Supplement the medium with 10% FBS Dissociation Reagent: Accutase Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820700a Required Product 3: 860015.0 Required Product 4: 830100.0