MOLT-4 Cells

Categories: Leukemia cell lines Description: MOLT-4 is a T lymphoblast cell line derived from the peripheral blood of a 19-year-old male patient with Acute lymphoblastic leukemia (ALL) in relapse in 1971. It is a sister cell line of MOLT-3, while MOLT-4 shows an unusual T-cell antigen receptor gamma-chain gene (T-gamma) rearrangement. MOLT-4 cells have a doubling time of around 30 hours, grow in suspension, and are tumorigenic in untreated nude mice, anti-lymphocyte serum-treated mice, and x-irradiated mice. MOLT-4 cells have a hypertetraploid chromosome number with the modal chromosome number of 95 occurring in 24% of cells but show stable and recurrent structural abnormalities of chromosomes and longer telomere length. MOLT-4 expresses a variety of T cell markers including CD1, CD2, CD3A, CD3B, CD3C, CD4, CD5, CD6, and CD7. They also express high levels of terminal deoxynucleotidyl transferase (TdT). The MOLT-4 cell line does not produce immunoglobulin or Epstein-Barr virus. The patient from whom the cells were derived had received prior multidrug chemotherapy. There is a G -> A mutation at codon 248 of the p53 gene, and P53 is not expressed. The line was initially contaminated with mycoplasma but has since been cured with antibiotics. Organism: Human Tissue: Peripheral blood Disease: Adult T acute lymphoblastic leukemia Synonyms: Molt-4, MOLT 4, Molt 4, MOLT.4, MOLT4, Molt4, GM02219, GM02219C, GM2219C, GM02219D Age: 19 years Gender: Male Ethnicity: Caucasian Morphology: Round cells Cell Type: T lymphocyte Growth Properties: Suspension Citation: MOLT-4 (Cytion catalog number 300115) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_0013 Protein Expression: p53 positive Antigen Expression: CD1 (49%), CD2 (35%), CD3 A (26%) B (33%) C (34%), CD4 (55%), CD5 (72%), CD6 (22%), CD7 (77%) Viruses: The cells do not produce immunoglobulin or Epstein-Barr virus (Minowada, 1972). Products: High levels of terminal deoxynucleotidyl transferase (TdT) are produced Mutational Profile: G -> A mutation at codon 248 of the p53 gene, P53 is not expressed (Rodrigues, 1990). Karyotype: Hypertetraploid. Modal number: 96. Two x and two Y chromosomes. Culture Medium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) Supplements: Supplement the medium with 10% FBS Subculturing: Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 105 cells/ml and keep the cell concentration within the range of 3 x 105 to 1 x 106 cells/ml for optimal growth. Seeding Density: 1 x 105 cells/cm2 Fluid Renewal: 2 to 3 times per week Post Thaw Recovery: 24 to 48 hours Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820700a Required Product 3: 860015.0 Required Product 4: 830100.0