Membrane VEGF CHO Cell Line

The Membrane VEGF CHO Cell Line is a clonal CHO cell line stably expressing a cleavage-resistant form of membrane-anchored human VEGF-A (vascular endothelial growth factor), that enforces its cell surface localization. VEGF-A belongs to the VEGF family of homodimer glycoproteins and is produced and secreted by various cells when angiogenesis is required. Angiogenesis involves endothelial cell proliferation, migration, and formation of blood vessels, which under normal conditions serve to provide nutrients and oxygen to tissues during development or wound healing. However, tumor cells can promote new blood vessel formation by secreting pro-angiogenesis factors. VEGF-A can bind to both VEGFR1 (Vascular Endothelial Growth Factor Receptor 1) and VEGFR2, also known as KDR (kinase insert domain receptor), on the surface of endothelial cells or cancer cells. The VEGF-VEGFR signal pathway has been a significant target in therapeutic strategies aimed at controlling angiogenesis in diseases like cancer and AMD (age macular degeneration), and several small molecules, neutralizing antibodies and blockers have been FDA-approved. Some VEGF isoforms exist as membrane-bound, and studies into their potential to generate ADCC (antibody-dependent cell mediated cytotoxicity) and CDC (complement-dependent cytotoxicity) are ongoing, making the development of cellular models where membrane-anchored VEGF is present crucial.