KG-1a Cells

Categories: Leukemia cell lines Description: The KG-1a cell line is a subline derived from the original KG-1 cell line, which was established from the bone marrow of a patient diagnosed with acute myeloid leukemia (AML). KG-1a cells are classified as a human myeloid leukemia cell line and are particularly characterized by their immature, undifferentiated state. Unlike the parent KG-1 cells, which are primarily in the myeloblast stage, KG-1a cells exhibit a more primitive phenotype, resembling early myeloid progenitors or even stem cells. This makes them an invaluable tool for studying hematopoiesis, leukemia progression, and the molecular mechanisms underlying myeloid differentiation. KG-1a cells express various surface markers typical of early hematopoietic progenitors, such as CD34, CD38, and HLA-DR, while lacking markers associated with mature myeloid cells. This profile makes them highly suitable for research into stem cell biology and the development of leukemia therapies. Additionally, KG-1a cells are often used in drug screening assays to evaluate the efficacy of potential anti-leukemic compounds, particularly those targeting leukemic stem cells. Their ability to maintain an undifferentiated state in vitro also provides a robust model for gene expression studies and functional assays related to leukemia pathogenesis. As with other cell lines derived from human tissue, KG-1a cells are intended for research use only and are not suitable for therapeutic or in vivo applications. They require careful handling under sterile conditions, and their growth characteristics necessitate specific culture conditions, including the use of RPMI-1640 medium supplemented with fetal bovine serum. Researchers utilizing the KG-1a cell line can gain significant insights into the early stages of leukemic transformation and the role of hematopoietic progenitors in cancer biology. Organism: Human Tissue: Bone marrow Disease: Acute myelogenous leukemia Synonyms: KG-1A, KG1A, KG1a Age: 59 years Gender: Male Ethnicity: Caucasian Cell Type: Myeloblast Growth Properties: Suspension Citation: KG-1a (Cytion catalog number 300234) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_1824 Antigen Expression: HLA A30, A31, B35, Cw4 Isoenzymes: G6PD, B, PGM1, 1-2, PGM3, 0, ES-D, 1, Me-2, 1, AK-1, 0, GLO-1, 2 Viruses: EBNA (EBNA ): negative Reverse Transcriptase: Negative Culture Medium: IMDM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 25 mM HEPES, w: 1.0 mM Sodium pyruvate, w: 3.024 g/L NaHCO3 (Cytion article number 820800a) Supplements: Supplement the medium with 10% FBS Doubling Time: 45 hours Subculturing: Transfer the cell suspension into sterile centrifuge tubes. Collect the cells by spinning down at 300xg for 3 minutes. Discard the supernatant and resuspend the pelleted cells in fresh cell culture medium. Adjust to an optimal cell density between 1 - 3 x 105 cells/ml. Split the cells when a maximum cell density of 1 - 2 x 106 cells/ml is reached. Fluid Renewal: Every 3 days Post Thaw Recovery: Allow the cells to recover from the freezing process for at least 24 hours. Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820800a Required Product 3: 860015.0 Required Product 4: 830100.0