Jurkat E6.1 Cells

Categories: Leukemia cell lines Description: Jurkat E6.1 cells, a derivative clone of the Jurkat cell line, which originates from the peripheral blood of a 14-year-old boy with acute T-cell leukemia, are a pivotal resource in the field of tumor immunology and leukemia research. These cells exhibit rapid proliferation and a pronounced responsiveness to stimuli, crucial for studying T cell biology, including T cell receptor (TCR) signaling, activation, proliferation, and apoptosis. Characterized by mutations such as the TEL-JAK2 fusion gene, Jurkat E6.1 cells provide insights into the leukemia phenotype and the molecular mechanisms underlying T-cell leukemia. Jurkat E6.1 cells are commonly used to investigate the intracellular signaling pathways that are activated upon TCR engagement, such as the NF-kappaB pathway, MAPK pathways, and calcium signaling, which are crucial for T cell activation and function. The cell line's responsiveness to phorbol esters and agents targeting the T3 antigen makes it an invaluable tool for exploring the intricacies of T-cell activation, including the induction of Interleukin-2 (IL-2) production. This feature, combined with their abnormal karyotype, underscores the utility of Jurkat E6.1 cells in research focused on the immune synapse architecture and the signaling pathways that govern T-cell proliferation and function. Jurkat E6.1 cells' utility extends to the study of apoptosis, offering a model to investigate the effects of various compounds, including alkaloids extract from sources such as Tribulus terrestris, on cell death pathways. This aspect is particularly relevant for identifying potential therapeutic agents and understanding their mechanisms of action in T-cell leukemia. In summary, Jurkat E6.1 cells, with their unique characteristics and versatility, continue to be a cornerstone in the study of T-cell activation, signaling, and apoptosis. Organism: Human Tissue: Blood Disease: Acute T cell leukemia Metastatic Site: T lymphocyte Synonyms: JurkatE6-1, Jurkat E6-1, Jurkat, Clone E6-1, Jurkat Clone E6-1, Jurkat (clone E6-1), JURKAT E-6.1, JURKAT E-61, Jurkat-E6, Jurkat E6, J.E6-1, E6-1 Age: 14 years Gender: Male Morphology: Round cells Cell Type: Lymphoblast Growth Properties: Suspension Citation: Jurkat E6.1 (Cytion catalog number 300223) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_0367 Antigen Expression: CD3 Products: Interleukin-2 (interleukin 2, IL-2), interferon gamma Karyotype: Modal number = 46, range = 41 to 47, the karyotype is 46,xY,-2,-18, del(2)(p21p23), del(18)(p11.2) Culture Medium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) Supplements: Supplement the medium with 10% FBS Subculturing: Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 105 cells/ml and keep the cell concentration within the range of 3 x 105 to 1 x 106 cells/ml for optimal growth. Seeding Density: 1 x 105 cells/ml Fluid Renewal: Every 2 days Post Thaw Recovery: Fast Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820700a Required Product 3: 860015.0 Required Product 4: 830100.0