IL-5 Responsive Luciferase Reporter Ba/F3 Cell Line

The IL-5 Responsive Luciferase Reporter Ba/F3 Cell Line is a murine Ba/F3 cell line engineered to express both human IL-5RA (IL-5 receptor alpha, also known as CD125) (NM_175726.4) and human CSF2RB (common receptor beta chain also known as IL5RB or CD131) (NM_000395.3), separated by a self-cleaving P2A peptide. The construct was delivered by lentiviral transduction of the STAT5 Luciferase Reporter Ba/F3 Cell Line (#79772), which expresses a firefly luciferase reporter driven by the STAT5 (signal transducer and activator of transcription 5) response elements located upstream of the minimal TATA promoter. After activation by IL-5, the endogenous transcription factor STAT5 binds to the response elements, inducing transcription of the luciferase reporter. This cell line has been validated to respond to IL-5. Additional functional validation demonstrates that IL-5-induced luciferase activity can be inhibited by either anti-IL-5 or anti-IL-5R neutralizing antibodies. Figure 1: Illustration of the mechanism of IL-5 Luciferase Reporter Ba/F3 Cell Line. IL-5 binds to IL-5 receptor alpha subunit, facilitating its interaction with the common receptor beta chain (CSF2RB), and activating downstream JAK1/2 (Janus kinase 1/2) tyrosine kinases, which phosphorylate the transcription factor STAT5 (signal transducer and activator of transcription 5). STAT5 phosphorylation triggers the formation of a homodimer and translocation to the nucleus, where it can activate the transcription of the Firefly luciferase reporter driven by STAT5 response elements present in the promoter. Interested in screening and profiling inhibitors or blocking antibodies of IL-5 in a cell culture assay without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.