IL-11 Responsive Luciferase Reporter HEK293 Line

The IL-11 (Interleukin-11) Responsive Luciferase Reporter HEK293 Line is a HEK293 cell line engineered to express both human IL-11Ra (IL-11 receptor alpha) (NM_001142784.3) and human IL-6ST (IL-6 cytokine family signal transducer, also known as gp130) (NM_002184.4) separated by a self-cleaving P2A peptide. The construct was delivered by lentiviral transduction of STAT3 Luciferase Reporter HEK293 Cell Line (#79800-P), which express a firefly luciferase reporter driven by STAT3 response elements located upstream of the minimal TATA promoter. After activation by IL-11, the endogenous transcription factor STAT3 binds to the response elements, inducing transcription of the luciferase reporter gene. This cell line has been validated to respond to IL-11. Figure 1: Illustration of the mechanism of action in the IL-11 Responsive Luciferase Reporter HEK293 Cell Line.Interested in screening and profiling inhibitors, blocking antibodies, or activators of IL-11 signaling without the need to purchase and license the cell line? Check out our Cell Signaling Pathway Screening.