ID8 Cells

Categories: Mouse cell lines Description: The ID8 cell line is a widely utilized murine model derived from spontaneous transformation of C57BL/6 mouse ovarian surface epithelial (MOSE) cells. This cell line closely mimics human epithelial ovarian cancer, making it a vital tool for preclinical research into ovarian cancer pathophysiology and treatment. ID8 cells are known for their ability to grow intraperitoneally in immunocompetent C57BL/6 mice, facilitating studies of tumor progression and metastasis. This model is particularly relevant for examining peritoneal tumor formation and ascites development, which are key features of advanced ovarian cancer in patients. ID8 cells exhibit the capability to form tumors when injected intraperitoneally, leading to disseminated cancer throughout the abdominal cavity and ascitic fluid accumulation. These properties enable the exploration of tumor-host interactions, including the role of the immune system and tumor microenvironment in cancer progression. In studies involving immunotherapies or combined treatment approaches, ID8 has proven valuable for evaluating the effects of interventions such as chemotherapy agents like carboplatin and immune checkpoint inhibitors targeting PD-L1. Research involving ID8 models has shown their utility in examining the influence of tumor metabolism on immune cell behavior, particularly macrophage polarization and function. For instance, tumors induced by ID8 cells can modulate the metabolism of peritoneal macrophages, altering their oxidative phosphorylation (OXPHOS) and promoting tumor growth through metabolic crosstalk. These insights have paved the way for exploring targeted metabolic therapies that may inhibit tumor-promoting immune cell adaptations. Organism: Mouse Tissue: Ovary Disease: Normal Synonyms: ID-8, ID8/MOSEC Breed: C57BL/6 Age: Adult Gender: Female Morphology: Epithelial-like Cell Type: Epithelial cell Growth Properties: Adherent Citation: ID8 (Cytion catalog number 305305) Biosafety Level: 1 Ncbi_ Taxid: 10090.0 Cellosaurus Accession: CVCL_IU14 Culture Medium: DMEM, w: 4.5 g/L Glucose, w: 4 mM L-Glutamine, w: 3.7 g/L NaHCO3, w: 1.0 mM Sodium pyruvate (Cytion article number 820300a) Supplements: Supplement the medium with 10% FBS Dissociation Reagent: Accutase Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820300a Required Product 3: 860015.0 Required Product 4: 830100.0