Human IL-15 Responsive Luciferase Reporter Ba/F3 Cell Line

The Human IL-15 Responsive Luciferase Reporter Ba/F3 Cell Line is a murine Ba/F3 cell line engineered to express both human CD122 (hIL2RB, NM_000878.5) and CD132 (hIL2RG, NM_000206.3) separated by a self-cleaving P2A peptide. The construct was delivered by lentiviral transduction of STAT5 Luciferase Reporter Ba/F3 cells (#79772), which express a firefly luciferase reporter driven by STAT5 (Signal transducer and activator of transcription 5) response elements located upstream of the minimal TATA promoter. After activation by IL-15, the endogenous transcription factor STAT5 binds to the response elements, inducing transcription of the luciferase reporter. This cell line has been validated to respond to human IL-15 and IL-2. Functional validation demonstrates that IL-15-induced luciferase activity can be inhibited by anti-IL15 neutralizing antibody Ordesekimab. Figure 1: Illustration of the mechanism of Human IL-15 Luciferase Reporter Ba/F3 Cell Line. IL-2 (interleukin 2) and IL-15 (interleukin 15) both bind to and signal through a complex composed of IL-2/IL-15 receptor beta chain (CD122) and the common gamma-chain (gamma-C, CD132). Activation of the receptor activates downstream JAK (janus kinase) tyrosine kinases, which phosphorylate the transcription factor STAT5. STAT5 phosphorylation triggers the formation of a homodimer and translocation to the nucleus, where it can activate the transcription of the Firefly luciferase reporter driven by STAT5 response elements present in the promoter.