HL-60 Cells

Categories: Leukemia cell lines Description: HL-60 cells, originating from a 36-year-old female with acute promyelocytic leukemia, serve as a vital model in cancer research, particularly in studying hematological malignancies, due to their ability to differentiate into mature white blood cells and mimic innate immune responses, aiding in the understanding of leukemic progression, cellular oncogene expression, and therapeutic target identification. The ability of HL-60 cells to differentiate into mature white blood cells, such as granulocytes and monocytes, through agents like dimethyl sulfoxide (DMSO) or retinoic acid, underscores their significance in studies related to human myeloid cell differentiation and sheds light on the mechanisms underpinning leukemic progression and the efficacy of therapeutic interventions. HL-60 human myeloid leukemia cells are integral to research focusing on apoptosis, cell activation, and the cell cycle, including the regulation of key oncogenes like the c-myc proto-oncogene and tumor necrosis factor (TNF-alpha). Their capability to form extracellular traps, structures involved in trapping and killing pathogens, which mirrors the innate immune response seen in primary neutrophils, makes HL-60 cells a useful model for studying the immune aspects of leukemia and how leukemic cells interact with the immune system. Moreover, the responsiveness of HL-60 cells to signaling pathways such as the MAPK pathway and various kinases is crucial for dissecting the molecular mechanisms driving leukemic cell proliferation and differentiation. This aspect is particularly beneficial for identifying therapeutic targets and developing new treatment strategies for leukemia. HL-60 cells are a critical resource in cancer research, offering insights into hematological malignancies, leukemic progression, and potential therapeutic targets through their unique differentiation capabilities and mimicry of immune responses. Organism: Human Tissue: Blood Disease: Acute promyelocytic leukemia Synonyms: HL 60, HL.60, HL60 Age: 36 years Gender: Female Ethnicity: Caucasian Morphology: Round cells Cell Type: Lymphoblast Growth Properties: Suspension Citation: HL-60 (Cytion catalog number 300209) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_0002 Receptors Expressed: complement, Fc Isoenzymes: G6PD, B, PGM1, 1, PGM3, 1, ES-D,1, Me-2, 1, AK-1, 1, GLO-1, 1 Oncogenes: myc+ Reverse Transcriptase: negative Products: tumor necrosis factor (TNF), also known as tumor necrosis factor alpha (TNF-alpha, TNF alpha), after stimulation with phorbol myristic acid Culture Medium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) Supplements: Supplement the medium with 10% heat-inactivated FBS Subculturing: Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 105 cells/ml and keep the cell concentration within the range of 3 x 105 to 1 x 106 cells/ml for optimal growth. Seeding Density: 2 x 105 cells/ml Fluid Renewal: 2 to 3 times per week Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820700a Required Product 3: 860015.0 Required Product 4: 830100.0