HiPSC-derived Endothelial Cells

Human Endothelial Cells (HiPSC-EC) from ScienCell Research Laboratories are differentiated from a human induced pluripotent stem cell line (HiPSC), which is generated using mRNA reprogramming technology from Human Dermal Fibroblasts (HDF). The monolayer HiPSC efficiently generate mesoderm cells via small-molecule activation of WNT signaling in chemically defined media, and subsequently converted to endothelial cells through endothelial cell specification using vascular endothelial growth factor (VEGF) and fibroblast growth factor (FGF). The derived endothelial cells are characterized by immunofluorescence with antibodies specific to CD31 and vWF. The cell population is highly pure: >80% of cells express CD31 (PECAM-1) & vWF positive. HiPSC-EC are cryopreserved at passage one and delivered frozen. Each vial contains >1 x 10 6 cells in 1 ml volume. Cells are negative for mycoplasma, bacteria, yeast and fungi. After reviving, HiPSC-EC can be maintained in Endothelial Cell Medium as an adherent culture. HiPSC-EC are not recommended for expanding or long-term cultures. Recommended Medium It is recommended to use the provided Endothelial Cell Medium (ECM, Cat. #1001) for plating HiPSC-EC and culturing them.