Firefly Luciferase NCI-H929 Cell Line

Firefly Luciferase NCI-H929 Cell Line is a NCI-H929 B lymphocyte cell line that constitutively expresses the firefly (Photinus pyralis) luciferase reporter under the control of a EF1A promoter. This cell line was generated by transduction with Firefly Luciferase Lentivirus (#78740-H). This cell line has been validated for luciferase activity. NCI-H929 is a B lymphocyte cell line isolated from a patient with plasmacytoma myeloma. It is a cell line with a B-cell plasma phenotype, able to secrete immunoglobulins. It was shown to express PCA-1 (plasma cell antigen 1), CD38 and the transferrin receptor. It does not seem to show expression of HLA -DR (human leukocyte antigen- DR isotype). This cell line has a rearrangement in the MYC gene and an activated RAS allele, and is a commonly used model in drug screening and as a CDX (cell line-derived xenograph) tumor model. The presence of firefly luciferase allows for easy readouts when performing cellular assays or animal experiments.