DMS-114 Cells

Categories: Lung cancer cell lines Description: DMS-114 is a human small-cell lung cancer (SCLC) cell line with unique features distinguishing it from other SCLC subtypes. Recent research has indicated that DMS-114, previously classified within the YAP1-expressing SCLC category (SCLC-Y), harbors pathogenic mutations in SMARCA4, an ATPase subunit of the SWI/SNF chromatin-remodeling complex. These mutations are associated with the absence of RB1 mutations, contrary to the typical mutational landscape of SCLC, which commonly features concurrent TP53 and RB1 alterations. This cell line's profile includes reduced expression of SMARCA4 mRNA and protein, contributing to its reclassification as a SMARCA4-deficient undifferentiated tumor (SMARCA4-UT) rather than a traditional SCLC. Morphological assessments have shown that DMS-114 aligns more closely with thoracic SMARCA4-UT, exhibiting traits such as lower neuroendocrine marker expression and a distinctive immunohistochemical profile. The revised classification of DMS-114 as a SMARCA4-deficient malignancy rather than SCLC has significant implications for its use as a preclinical model. It serves as an important resource for studying therapeutic strategies targeting SMARCA4-related pathways and investigating the biology of aggressive thoracic cancers that mimic SCLC. Unlike conventional SCLC, SMARCA4-deficient tumors, including DMS-114, often present with unique gene expression profiles marked by high YAP1 expression, loss of certain neuroendocrine markers, and distinct molecular vulnerabilities. This insight underscores the necessity of comprehensive molecular and histopathological analysis for accurate tumor classification and the development of effective treatment strategies. Organism: Human Tissue: Lung Disease: Thoracic SMARCA4-deficient undifferentiated tumor Synonyms: DMS-114, DMS114, Darmouth Medical School 114 Age: 68 years Gender: Male Ethnicity: Caucasian Growth Properties: Adherent Citation: DMS-114 (Cytion catalog number 305364) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_1174 Receptors Expressed: Epidermal growth factor (EGF), complement (CR3) Protein Expression: Genes expressed: adrenocorticotropin (adrenocorticotropic hormone, ACTH), bombesin, glucagon, 17 beta estradiol, oxytocin - neurophysin (OT-NP) Antigen Expression: Leu 7 +, My23 +, CD11b + Tumorigenic: Yes, in nude mice Mutational Profile: Mutation: SMARCA4, p.Glu1310Ter (c.3928G>T), homozygous, Mutation: PARD3B, Ex2-14del, homozygous, Mutation: TP53, p.Arg213Ter (c.637C>T), homozygous Culture Medium: Waymouth's MB 752/1 medium (We do not supply this product, please consider other suppliers. Please let us know if you need further assistance) Supplements: Supplement the medium with 10% FBS Dissociation Reagent: Accutase Fluid Renewal: 2 times per week Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 3: 860015.0 Required Product 4: 830100.0