COS-7 Cells

Categories: Monkey cell lines Description: COS-7 cells are a fibroblast-like cell line derived from African green monkey kidney tissue and are a vital resource in research, particularly for their high transfection efficiency, making them a popular choice for the expression of recombinant proteins. COS-7 cells are derived from the CV-1 cell line and transformed with a mutant form of the simian virus 40 (SV40), which includes a replication origin that allows for episomal replication of transfected plasmids containing the SV40 origin of replication. Transfection of COS-7 cells is facilitated by transfection reagents such as Lipofectamine, with an efficiency that mirrors those observed in HeLa cells. Conventional methods can achieve up to 80% transfection efficiency in COS-7 cells, showcasing their ease of genetic manipulation. The ability of COS-7 cells to accommodate large plasmids and replicate them, leading to high yields of the desired recombinant proteins, make them an invaluable resource for various applications, including gene expression studies, signal transduction pathway investigations, and the production of proteins for biochemical analyses. COS-7 cells exhibit a strong susceptibility to various viruses, making them an excellent model for virology studies, including virus-host interaction investigations, viral life cycle elucidation, and antiviral drug testing. Their permissiveness to viral entry and replication is leveraged to study the mechanisms of viral infection, pathogenesis, and the cellular responses elicited by viral invaders. Consequently, COS-7 cells serve as a valuable tool in the development of viral vectors for gene therapy and vaccine research. COS-7 cells are a cornerstone in research due to their high transfection efficiency and utility in recombinant protein expression. Their ease of genetic manipulation, combined with susceptibility to viruses, makes them indispensable for studies in gene expression, signal transduction, virology, and the development of viral vectors, solidifying their role as a versatile tool in both basic and applied biological sciences. Organism: Cercopithecus aethiops (Green monkey) Tissue: Kidney Synonyms: Cos-7, COS7, Cos7, CV-1 in Origin Simian-7 Age: Adult Gender: Male Morphology: Fibroblast-like Cell Type: Fibroblast Growth Properties: Monolayer, adherent Citation: COS-7 (Cytion catalog number 605470) Biosafety Level: 1 Ncbi_ Taxid: 9534.0 Cellosaurus Accession: CVCL_0224 Virus Susceptibility: SV40 (lytic growth), SV40 tsA209 at 40 degree Celsius, SV40 mutants with deletions in the early region Products: T antigen Culture Medium: DMEM:Ham's F12 (1:1), w: 3.1 g/L Glucose, w: 2.5 mM L-Glutamine, w: 15 mM HEPES, w: 0.5 mM Sodium pyruvate, w: 1.2 g/L NaHCO3 (Cytion article number 820400a) Supplements: Supplement the medium with 10% FBS Dissociation Reagent: Accutase Subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. Seeding Density: 1 x 104 cells/cm2 will yield in a confluent layer in about 4 days Fluid Renewal: 2 to 3 times per week Post Thaw Recovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Required Documentation: A CITES (Convention on International Trade in Endangered Species of Wild Fauna and Flora) certificate is required to export this article to your country. CITES oversees trade in specific species globally to ensure their protection and sustainability. Our company, as the exporter, will handle the CITES certificate application on your behalf. The process typically takes 2-4 weeks and incurs a fee of 350?. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Disclaimer: Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Certificate Of Analysis: The certificate of analysis can be requested on the website or via email at info@cytion.com. Please indicate the lot number of your product in the email. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820400a Required Product 3: 860015.0 Required Product 4: 830100.0