CHO Cells

Categories: Hamster cell lines Description: Chinese hamster ovary (CHO) cells are a cornerstone in the field of biotechnology and are heavily utilized in the process of CHO cell line development for the manufacture of biopharmaceuticals. These include monoclonal antibodies, recombinant antibody expression, and vaccines. The many advantages of CHO cells underscore their popularity in biomanufacturing, positioning them as a robust and versatile animal cell line with a proven track record in genetics, molecular biology, toxicity screening, nutrition, and gene expression studies. The contribution of CHO cells to the biopharmaceutical industry is immense, with their role in the development of recombinant antibodies and monoclonal antibody production being particularly significant. Nearly 50 biotherapeutics developed using these cells have been approved in the USA and EU, which speaks to the efficacy of CHO cells and their integral role in antibody development. Their hamster origin contributes to lower susceptibility to viruses, enhancing biosafety in biomanufacturing settings and reducing batch-to-batch variation. CHO cells are well-suited to produce proteins that undergo post-translational modifications, which is critical for therapeutic protein production. The versatility of the Chinese Hamster Ovary-derived cells is further highlighted by their fast proliferation rates and high protein expression rates of 1-5 grams per liter of culture. The ease of cultivating CHO cells and their ability to be genetically modified makes CHO cells an optimal choice for both transient and stable expression studies. The CHO-K1 cell line, a derivative of the original Chinese hamster ovary (CHO) cells is frequently utilized in expressing recombinant proteins, especially for the production of therapeutic proteins and recombinant antibodies. They excel in producing therapeutic proteins and antibodies due to efficient post-translational modification, notably glycosylation. Researchers modify CHO-K1 cells to enhance protein expression and tailor glycosylation for specific therapies, crucial in biomedicine. In conclusion, the Chinese hamster ovary cell line, known for its remarkable ability to mimic human post-translational modifications, is an invaluable scientific resource. Whether overcoming the difficulty of expressing challenging proteins or monoclonal antibody production, CHO cells have revolutionized the development and production of recombinant protein therapeutics. They remain pivotal in modern medicine, serving as a cornerstone for biopharmaceutical production and reflecting the advancements in biotechnology. Organism: Chinese hamster Tissue: Ovary Synonyms: Chinese Hamster Ovary, CHO-ori Age: Adult Gender: Female Morphology: Epithelial-like Growth Properties: Monolayer, adherent Citation: CHO (Cytion catalog number 603479) Biosafety Level: 1 Ncbi_ Taxid: 10029.0 Cellosaurus Accession: CVCL_0213 Culture Medium: Ham's F12, w: 1.0 mM stable Glutamine, w: 1.0 mM Sodium pyruvate, w: 1.1 g/L NaHCO3 (Cytion article number 820600a) Supplements: Supplement the medium with 10% FBS Dissociation Reagent: Accutase Subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. Seeding Density: 3 x 104 cells/cm2 will yield in a confluent layer in about 4 days Fluid Renewal: 2 to 3 times per week Post Thaw Recovery: After thawing, plate the cells at 5 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 hours. Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Disclaimer: Our cells are provided for in vitro laboratory research purposes exclusively and are not intended for clinical or diagnostic use, nor are they to be administered to humans or used for veterinary purposes. Users must adhere to all applicable guidelines and regulations for the handling and use of these cells in a research setting. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Certificate Of Analysis: The certificate of analysis can be requested on the website or via email at info@cytion.com. Please indicate the lot number of your product in the email. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820600a Required Product 3: 860015.0 Required Product 4: 830100.0