CCRF-CEM Cells

Categories: Leukemia cell lines Description: CCRF-CEM cells are a type of human T lymphoblasts commonly used in immuno-oncology and immunology research. These cells were isolated from the peripheral blood of a 4-year-old female Caucasian with acute lymphoblastic leukemia (ALL). CCRF-CEM grow in suspension and can reach high cell density when cultured in spinner flasks. Karyotype analysis of CCRF-CEM cells showed a modal number of 47 chromosomes, ranging from 41 to 95. They show no consistent loss or gain of specific chromosomes and no marker chromosomes. However, 28% of cells with 45 chromosomes showed C- and 53% of all cells had an extra D, and 35% had an additional F. CCRF-CEM cells are tumorigenic and can cause tumours in Syrian hamsters. These cells express CD3, CD5, CD7, and CD4 genes and antigens. Additionally, isoenzyme analysis showed ADA, 1, ES-D, 1, G6PD, B, GLO-I, 1, PEP-D, 1, PGD, C, PGM1, 1, PGM3, 0. These cells are reported to be free of virus particles as determined by electron microscopy. A study has shown that the combination of resveratrol and prednisolone induced apoptosis in CCRF-CEM cells in a time- and dose-dependent manner. The combination treatment showed synergistic effects on the overexpression of BAx and the downregulation of BCL2. Organism: Human Tissue: Peripheral blood Disease: Leukemia Synonyms: CCRF/CEM, CCRFCEM, CCRF.CEM, CCRF CEM, CCRF, CEM, CEM-CCRF, CEM-CCRF (CAMR), CCRF/CEM/0, CEM/0, CEM-0, CCRF-CEM/S, GM03671, GM03671C Age: 4 years Gender: Female Ethnicity: Caucasian Morphology: Polymorph cells, big nuclei, formation of microvilli Cell Type: T lymphoblast Growth Properties: Suspension Citation: CCRF-CEM (Cytion catalog number 300147) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_0207 Protein Expression: p53 negative Antigen Expression: CD3 B (37%), CD4 (50%), CD5 (95%), CD7 (77%) Isoenzymes: G6PD, B Tumorigenic: Yes, in nude mice Viruses: EBV negative Reverse Transcriptase: negative Ploidy Status: Aneuploid Msi Status: Instable (MSI) Culture Medium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) Supplements: Supplement the medium with 10% heat-inactivated FBS Doubling Time: 24 hours Subculturing: Maintain cultures by periodically adding or replacing the medium. Initiate cultures with a density of 5 x 105 cells/ml and keep the cell concentration within the range of 3 x 105 to 1 x 106 cells/ml for optimal growth. Seeding Density: Start new cultures at 1 x 105 cells/ml Fluid Renewal: Every 3 days Post Thaw Recovery: Allow the cells to recover from the freezing process for at least 48 hours. Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820700a Required Product 3: 860015.0 Required Product 4: 830100.0