B-LCL-HROC285 Cells

Categories: B lymphoblast cell lines , HROC cell lines Description: B-LCL-HROC285 is an Epstein-Barr virus (EBV) transformed B lymphocyte cell line derived from a patient, who had colon adenocarcinoma associated with Lynch syndrome. This specific type of colon cancer is linked to hereditary non-polyposis colorectal cancer (HNPCC), commonly caused by mutations in DNA mismatch repair genes. The B-LCL-HROC285 cell line allows for the study of EBV-related transformation processes in B cells, as well as insights into cancer-related immune responses. The B-LCL-HROC285, provides a valuable tool for understanding the immune system's interactions with cancer cells, particularly how transformed B cells might interact with the immune environment in colorectal cancers arising from Lynch syndrome. This cell line is useful for immunological and oncological studies due to its genetic background and the EBV transformation process, which is known to influence B-cell proliferation and clonal selection. Organism: Human Tissue: Colon Disease: Adenocarcinoma Synonyms: B-LCL CO285, Bc HROC285 Age: 30 years Gender: Female Ethnicity: Caucasian Morphology: Round cells Cell Type: B lymphoblast Growth Properties: Suspension Citation: B-LCL-HROC285 (Cytion catalog number 300869) Biosafety Level: 2 Ncbi_ Taxid: 9606.0 Viruses: Transformant: EBV Culture Medium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) Supplements: Supplement the medium with 10% heat-inactivated FBS Subculturing: Gently homogenize the cell suspension in the flask by pipetting up and down, then take a representative sample to determine the cell density per ml. Dilute the suspension to achieve a cell concentration of 1 x 105 cells/ml with fresh culture medium, and aliquot the adjusted suspension into new flasks for further cultivation. Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Third- Party Agreements: Please note that this cell line is not available under a standard Cytion MTA, as it requires a third-party agreement and/or is subject to negotiation with the original licensor. Required Product 1: 820700a Required Product 3: 860015.0 Required Product 4: 830100.0