AP-1 Reporter-HEK293 Cell Line (JNK signaling pathway), 1,5x 10(6) cells

The stress-activated protein kinase/c-jun N-terminal kinase (SAPK/JNK) family of proteins incluces mitogen-activated protein kinases (MAPKs) that are activated by stress, inflammatory cytokines, mitogens, oncogenes, and inducers of cell differentiation and morphogenesis. Upon activation of the SAPK/JNK pathway, MAP Kinase Kinases phosphorylate and activate JNKs. The activated JNKs translocate to the nucleus where they phosphorylate and activate transcription factors such as c-Jun. cJun then binds to the activator protein-1 (AP1) response element and induces AP1 transcription. The AP1 Reporter-HEK293 cell line contains a firefly luciferase gene under the control of AP1-responsive elements that are stably integrated into HEK293 cells. This cell line is validated for its response to stimulation by Phorbol 12-Myristate 13-Acetate (PMA) and to treatment with inhibitors of the JNK signaling pathway. Applications: Monitor the JNK signaling pathway activity and AP1-mediated activity and screen for activators or inhibitors of the JNK signaling pathway. Format: Each vial contains about 1.5 X 10(6) cells in 1 ml of 10% DMSO. References: 1. Zhou H. et. al. (2005) Frequency and distribution of AP-1 sites in the human genome. DNA Research. 11: 139-150. 2. Gaillard P. et.al. (2005) Design and synthesis of the first generation of novel potent, selective, and in vivo active (benzothiazol-2-yl)acetonitrile inhibitors of the c-Jun N-terminal kinase. J Med Chem. 48(14):4596-4607.