Anti-GPC3 CAR-T Cells

Anti-GPC3 CAR-T Cells are produced by high-titer lentiviral transduction of human primary CD4+ and CD8+ T cells using Anti-GPC3 CAR Lentivirus (Clone GC33-CD28TM-41BB-CD3zeta) (BPS Bioscience #82494). These ready-to-use CAR-T cells express an anti-GPC3 (glypican-3) CAR (chimeric antigen receptor) consisting of the ScFv portion of anti-GPC3 (clone GC33) linked to a 2nd generation CAR, containing IgG1 short hinge and CD28 transmembrane domains, 4-1BB and CD3zeta signaling domains (Figure 1). These CAR-T cells have been validated using flow cytometry (to determine CAR expression) and co-culture cytotoxicity assays. Figure 1. Construct diagram showing components of the Anti-GPC3 CAR expressed in Anti-GPC3 CAR-T Cells.