Anti-CD19 CAR-T Cells (eGFP)

Anti-CD19 CAR-T Cells (eGFP) were produced by high-titer lentiviral transduction of human primary CD4+ and CD8+ T cells with SIN Anti-CD19 CAR Lentivirus (CD19 ScFv-CD8-4-1BB-CD3zeta eGFP) (#78775). These ready-to-use CAR (Chimeric Antigen Receptor)-T cells express an anti-CD19 CAR consisting of the ScFv (Single chain fragment variable) of anti-CD19 (clone FMC63) linked to a 2nd generation CAR containing CD8 hinge and transmembrane domains, and the 4-1BB and CD3zeta signaling domains (Figure 1). The presence of eGFP (enhanced green fluorescent protein) allows for easy fluorescent detection of CAR-expressing cells. These CAR-T cells have been validated by flow cytometry (to determine the CAR expression) and co-culture cytotoxicity assays.