A498 Cells

Categories: Kidney cancer cell lines Description: A498 cells are a human renal cell carcinoma cell line derived from the kidney tissue of a 58-year-old Caucasian male. These cells are extensively used in research related to kidney cancer, particularly for studying clear cell renal cell carcinoma, which is the most common type of kidney cancer in adults. The A498 cell line is characterized by its epithelial-like morphology and has been a valuable model for investigating the molecular and cellular mechanisms of renal carcinogenesis. These cells exhibit several features typical of kidney cancer, including alterations in the expression of genes involved in cell cycle regulation, apoptosis, and angiogenesis. A498 cells are particularly useful for examining the metabolic pathways altered in kidney cancer, as they display a distinct metabolic profile that includes changes in lipid and glucose metabolism. This aspect makes them suitable for metabolic targeting studies, which explore how altering metabolic pathways can inhibit tumor growth. Furthermore, A498 cells are employed in drug discovery and toxicology studies to test the efficacy of new chemotherapeutic agents and targeted therapies. They are also used to study the response of renal cancer cells to hypoxic conditions-a common feature of solid tumors that significantly influences tumor behavior and treatment response. Overall, the A498 cell line serves as an essential tool in renal cancer research, facilitating the development of more effective therapeutic strategies and enhancing our understanding of kidney cancer biology. Organism: Human Tissue: Kidney Disease: Renal cell carcinoma Synonyms: A-498 Age: 52 years Gender: Male Ethnicity: Caucasian Morphology: Epithelial-like Growth Properties: Monolayer, adherent Citation: A498 (Cytion catalog number 300113) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_1056 Isoenzymes: PGM3, 1, PGM1, 1-2, ES-D, 2, Me-2, 1, AK-1, 1, GLO-1, 2, G6PD, B Tumorigenic: Yes, in nude mice. Forms undifferentiated carcinoma, also forms tumors in anti thymocyte serum treated newborn mice Ploidy Status: Bimodal, tetraploid Msi Status: Stable (MSS) Culture Medium: EMEM (MEM Eagle), w: 2 mM L-Glutamine, w: 2.2 g/L NaHCO3, w: EBSS (Cytion article number 820100a) Supplements: Supplement the medium with 10% FBS and 1% NEAA Dissociation Reagent: Accutase Doubling Time: 62 hours Subculturing: Remove the old medium from the adherent cells and wash them with PBS that lacks calcium and magnesium. For T25 flasks, use 3-5 ml of PBS, and for T75 flasks, use 5-10 ml. Then, cover the cells completely with Accutase, using 1-2 ml for T25 flasks and 2.5 ml for T75 flasks. Let the cells incubate at room temperature for 8-10 minutes to detach them. After incubation, gently mix the cells with 10 ml of medium to resuspend them, then centrifuge at 300xg for 3 minutes. Discard the supernatant, resuspend the cells in fresh medium, and transfer them into new flasks that already contain fresh medium. Seeding Density: 1 x 104 cells/cm2 will result in a confluent monolayer within 4 days. Fluid Renewal: Every 3 days Post Thaw Recovery: After thawing, plate the cells at 2 x 104 cells/cm2 and allow the cells to recover from the freezing process and to adhere for at least 24 to 48 hours. Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820100a Required Product 3: 860015.0 Required Product 4: 830100.0