A2780 Cells

Categories: Reproductive system cancer cell lines Description: A2780 is a human ovarian cancer cell line that was first established in 1972 from a patient with advanced epithelial ovarian cancer. The cells were characterized as being sensitive to cisplatin and doxorubicin, two commonly used chemotherapy drugs for ovarian cancer. Since its establishment, A2780 has been widely used in cancer research studies, particularly in the development and testing of new cancer treatments. Research using A2780 cells has provided valuable insights into the biology of ovarian cancer, including the identification of specific genetic mutations such as TP53 and BRCA1. These mutations are associated with increased risk of ovarian cancer and are also found in other types of cancer. Additionally, A2780 cells have been used to study the role of angiogenesis, the process by which new blood vessels form, in ovarian cancer progression and to evaluate the efficacy of anti-angiogenic drugs. Angiogenesis plays a critical role in the growth and progression of ovarian cancer as it provides oxygen and nutrients for the cancer cells to grow. Studies using A2780 cells have demonstrated the overexpression of pro-angiogenic factors such as VEGF and angiopoietin-2, which promote the formation of new blood vessels. Additionally, A2780 cells have been used to test the efficacy of anti-angiogenic drugs such as bevacizumab, which target VEGF and inhibit the formation of new blood vessels. Furthermore, A2780 cells have been used to evaluate the efficacy of various therapeutic agents, including chemotherapy drugs, targeted therapies such as PARP inhibitors, and immunotherapies. In particular, A2780 cells have been used to study the effect of different drug combinations on cancer cell proliferation, apoptosis, and drug resistance. Overall, the A2780 cell line has played a significant role in the advancement of ovarian cancer research, providing a valuable tool for understanding the disease and developing new treatments. Organism: Human Tissue: Ovary Synonyms: A-2780, 2780, A2780S Age: Unspecified Gender: Female Growth Properties: Adherent/suspension Citation: A2780 (Cytion catalog number 300491) Biosafety Level: 1 Ncbi_ Taxid: 9606.0 Cellosaurus Accession: CVCL_0134 Culture Medium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) Supplements: Supplement the medium with 10% FBS Dissociation Reagent: Accutase Subculturing: Gather the suspension cells in a 15 ml tube and gently wash the adherent cells with PBS lacking calcium and magnesium (use 3-5 ml for T25 flasks and 5-10 ml for T75 flasks). Apply Accutase (1-2 ml for T25 flasks, 2.5 ml for T75 flasks) ensuring full coverage of the cell layer. Allow the cells to incubate at room temperature for 10 minutes. Following incubation, combine and centrifuge both the suspension and adherent cells. After centrifugation, carefully resuspend the cell pellet and transfer the cell suspension into new flasks containing fresh medium. Fluid Renewal: 2 to 3 times per week Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820700a Required Product 3: 860015.0 Required Product 4: 830100.0