A20 Cells

Categories: Mouse cell lines Description: The A20 cell line is derived from a reticulum cell sarcoma in a mouse and is widely used in immunology and cancer research. Reticulum cell sarcoma is a type of B-cell lymphoma, and A20 cells provide a valuable model for studying the biology of B-cell lymphomas and the immune response. These cells are particularly useful for investigating the mechanisms of B-cell development, activation, signaling, and the interaction between tumor cells and the immune system. Additionally, A20 cells play a crucial role in research focused on the production and function of cytokines, which are essential for immune regulation. A20 cells display a lymphoblastic morphology and express surface markers typical of B-cells, including surface immunoglobulin and major histocompatibility complex (MHC) molecules. Researchers utilize A20 cells to study antigen presentation, B-cell receptor signaling, and the role of various cytokines in immune responses. These cells are also instrumental in the development and testing of immunotherapies, such as monoclonal antibodies and checkpoint inhibitors, aimed at treating B-cell lymphomas and other hematological malignancies. Additionally, A20 cells serve as a model for evaluating the efficacy and safety of novel therapeutic agents in preclinical studies. The utility of A20 cells in immunological research and the understanding of B-cell pathophysiology highlights their importance in advancing cancer research and developing new treatment strategies. Organism: Mouse Disease: Mouse reticulum cell sarcoma Synonyms: A-20 Breed: BALB/cAnN Age: >15 months Gender: Unspecified Morphology: Lymphoblast Cell Type: B lymphocyte Growth Properties: Suspension Citation: A20 (Cytion catalog number 305263) Biosafety Level: 1 Ncbi_ Taxid: 10090.0 Cellosaurus Accession: CVCL_1940 Tumorigenic: Yes Culture Medium: RPMI 1640, w: 2.0 mM stable Glutamine, w: 2.0 g/L NaHCO3 (Cytion article number 820700a) Supplements: Supplement the medium with 10% heat-inactivated FBS, add 2.5 g/L glucose and 10 mM HEPES Subculturing: Suspension cells: Remove cells from substrate by pipetting with fresh medium. To obtain single cells, pass the suspension several times through a 22 gauge needle and dispense into new flasks. Freeze Medium: As a cryopreservation medium, use complete growth medium (including FBS) + 10% DMSO for adequate post-thaw viability, or CM-1 (Cytion catalog number 800100), which includes optimized osmoprotectants and metabolic stabilizers to enhance recovery and reduce cryo-induced stress. Thawing And Culturing Cells: Confirm that the vial remains deeply frozen upon delivery, as cells are shipped on dry ice to maintain optimal temperatures during transit. Upon receipt, either store the cryovial immediately at temperatures below -150°C to ensure the preservation of cellular integrity, or proceed to step 3 if immediate culturing is required. For immediate culturing, swiftly thaw the vial by immersing it in a 37°C water bath with clean water and an antimicrobial agent, agitating gently for 40-60 seconds until a small ice clump remains. Perform all subsequent steps under sterile conditions in a flow hood, disinfecting the cryovial with 70% ethanol before opening. Carefully open the disinfected vial and transfer the cell suspension into a 15 ml centrifuge tube containing 8 ml of room-temperature culture medium, mixing gently. Centrifuge the mixture at 300 x g for 3 minutes to separate the cells and carefully discard the supernatant containing residual freezing medium. Gently resuspend the cell pellet in 10 ml of fresh culture medium. For adherent cells, divide the suspension between two T25 culture flasks, for suspension cultures, transfer all the medium into one T25 flask to promote effective cell interaction and growth. Adhere to established subculture protocols for continued growth and maintenance of the cell line, ensuring reliable experimental outcomes. Sterility: Mycoplasma contamination is excluded using both PCR-based assays and luminescence-based mycoplasma detection methods. To ensure there is no bacterial, fungal, or yeast contamination, cell cultures are subjected to daily visual inspections. Safety Precautions: When planning to store a cryovial in liquid nitrogen for future thawing, it is mandatory to adhere to stringent safety measures. Appropriate protective gloves and clothing are essential, and the use of a face mask or safety goggles is required during the transfer of frozen samples to or from the liquid nitrogen tank. This is to mitigate the risk of injury from potential cryovial explosions upon removal, which can result in the projection of sharp fragments. Warranty: We stand by the promise of delivering products with high cell viability and robust culture performance. To achieve the best results, please make sure you follow the storage and culture instructions detailed in the product information sheet closely. Your adherence to these guidelines is key to success. Subject To Material Transfer Agreements: If you intend to use Cytion cell lines solely for internal research at a single research site, please complete and sign our Material Transfer Agreement (MTA) and submit it along with your order.For any commercial applications - including but not limited to fee-for-service work, quality control testing, product release, diagnostic use, or regulatory studies - please complete the Intended Use Form so we can prepare a suitable agreement tailored to your project.Please note: The MTA applies only to certain cell lines. If this notice and the MTA document appear on a product page, the agreement is applicable. For cell lines not covered by the MTA, no reference to the agreement will be shown. The MTA is not valid for customers in the Americas, China, or Taiwan. Please contact our U.S. entity to receive the appropriate agreement. Required Product 1: 820700a Required Product 3: 860015.0 Required Product 4: 830100.0