VCAM-1 BioAssay(TM) ELISA Kit, Mouse

Sandwich ELISA quantitative detection kit for Mouse VCAM-1 in cell culture supernates, serum and plasma (heparin, EDTA). Vascular cell adhesion molecule 1 (VCAM-1) is a cell surface glycoprotein adhesive for certain blood leukocytes and tumor cells, which is expressed by activated endothelium in a variety of pathologic conditions including atherosclerosis.Increased expression of VCAM1 is associated with a variety of chronic inflammatory conditions, making its expression and function a target for therapeutic intervention.Integrin alpha4beta1 (VLA-4) and VCAM-1 facilitate a critical cell-cell adhesion event required for survival of endothelial and mural cells during vascularization.The VCAM1 gene is assigned to the 1p31-32 region of chromosome 1 based on the analysis of human-mouse hybrid cell lines and in situ hybridization.The standard product used in this kit is recombinant mouse VCAM-1, a dimeric protein linking with a disulfide bond. It consists of Phe25-Glu698 amino acid sequence of a single chain with the molecular mass of 101.9kD. As a result of glycosylation, the molecular mass of 120kD is revealed by SDS-PAGE. , Assay Range:, 156pg/ml-10,000pg/ml, Sensitivity: < 5 pg/ml, Crossreactivity: There is no detectable cross-reactivity with other relevant proteins. Uniprot ID: P29533, Entrez Gene ID: 22329, Kit Components: 96-well plate pre-coated with anti-VCAM-1, Mouse, Lyophilized recombinant VCAM-1, Mouse standard, 10ng/tube, 2Ea, USB Cat No Kit Component, 143696 Avidin-Biotin-Peroxidase Complex (ABC), 130ul (1:100 dilution), 143697 Sample diluent buffer, 30ml, 143698 Antibody diluent buffer, 12ml, 143699 ABC diluent buffer, 12ml, 143700 TMB color developing agent, 10ml, 143701 TMB stop solution, 10ml, Storage and Handling: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles.